As the field of flow cytometry expands our knowledge of the immune system, increased panel complexity has put a greater demand on instrumentation. Spectral unmixing has advanced panel size and diversity, enabling the study of novel cell populations. To help meet these needs, we have developed the Invitrogen™ Attune™ Xenith™ Flow Cytometer to bring clog-resistant acoustic focusing technology to high parameter flow cytometry. This system enables the use of spectral unmixing and high parameter compensation at speeds up to 500 µL/minute with lnvitrogen™ CytKick™ Autosampler compatibility.