Use of the whole proteome of Giardia lamblia and immunoinformatic to identify immunogenic antigens

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Presentation Time: 03:15 PM - 04:30 PM
Poster Board Number: B954
Abstract ID: 6661

Presenting Author:
David Ortega-Tirado , Use of the whole proteome of Giardia lamblia and immunoinformatic to identify immunogenic antigens at Univ. de Sonora

Abstract:

Giardia lamblia is a protozoan responsible of the parasitosis known as giardiasis. This protozoan is classified into eight assemblages, named A-H. Assemblages A and B are responsible for human giardiasis. There is no vaccine against this human parasitosis, therefore, it is vital to know the main immunogenic antigens of G. lamblia. Our main goal was to identify immunogenic antigens from whole proteome of G. lamblia (A and B assemblages) by using immunoinformatic. FASTA sequences from GiardiaDB (https://giardiadb.org/giardiadb/app) were submitted to NetMHCII 2.3 (http://tools.iedb.org/mhcii/) of IEDB. The immunoinformatic analysis was performed against five murine MHC-II alleles: H2-IA^b, H2-IA^d, H2-IE^d, H2-IA^k, H2-IE^k. Python 3.9 was used to build a code that allowed the use of data science to analyze all protein sequences. We identified 501 proteins with immunogenic T-cell peptides for assemblage A, and 448 proteins for assemblage B. The proteins were distributed between all five MHC-II alleles. Most of the identified proteins had peptides sequences that predominantly joined to H2-IA^b and H2-IE^k. The H2-IA^k allele was the most restrictive MHC class II allele for both Giardia assemblages. Additionally, we also found proteins that belong to the G. lamblia secretome (14 for assemblage A and 12 for assemblage B). These results show all the potential immunogenic antigens of G. lamblia and contribute to the future rational development of a vaccine against giardiasis.