Gelatinases are important for CD4+ T-cell metabolic reprogramming.

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Presentation Time: 03:15 PM - 04:30 PM
Poster Board Number: B640
Abstract ID: 7846

Presenting Author:
Ella C Protz , Undergraduate Student at Florida Atlantic Univ.

Abstract:

Matrix metalloproteinases (MMP) are a family of proteolytic enzymes essential for various processes such as development and healing. Alternatively, the dysregulation of the gelatinases, MMP-2 and MMP-9, is implicated in immune cell migration and inflammation associated with multiple sclerosis (MS). MS is an autoimmune disease in which CD4+ T-cells play a key role both in humans and its animal model, experimental autoimmune encephalomyelitis (EAE). CD4+ T-cells are known to rely on both oxidative phosphorylation and aerobic glycolysis during various stages of activation. We have recently demonstrated that gelatinase inhibition can impact CD4+ T-cell activation and mitigate EAE progression. The present study utilized the Seahorse assay to characterize the cellular energetics of CD4+ T-cells in response to gelatinase inhibition. The resultant data suggests that gelatinases are important for both oxidative phosphorylation and the effective induction of glycolysis in CD4+ T-cells.