2-Deoxy-D-glucose as a potential therapeutic agent for reducing neuroinflammation following spinal cord injury

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Presentation Time: 03:15 PM - 04:30 PM
Poster Board Number: B660
Abstract ID: 4885

Presenting Author:
Stephane Vinit , Associate Professor at Univ. Paris Saclay

Abstract:

High spinal cord injuries (SCIs) lead to persistent diaphragm paralysis and related respiratory dysfunctions. The absence of functional recovery is due to limited neuroplasticity processes, mainly caused by chronic neuroinflammation occurring in the injured spinal cord. In this study, we aimed at reducing glucose metabolism that supports neuroinflammatory processes in an acute preclinical model of C2 spinal cord lateral hemi-section in rats. We administered 2-Deoxy-D-glucose (2-DG; 200 mg/kg/day s.c. for 7 days) and evaluated the effect on respiratory function and chondroitin sulfate proteoglycans (CSPGs) production around spinal phrenic motoneurons. Contrary to our initial hypothesis, our 2-DG treatment did not have any effect on diaphragm activity and CSPGs production in injured rats, even if a slight increase in tidal volume can be observed. Unexpectedly, it led to deleterious effects in uninjured (sham) animals, characterized by an increased ventilation and CSPGs production. Globally, our results seem to indicate that this 2-DG treatment paradigm could create a neuroinflammatory state in healthy animals, without altering or modulating the already installed spinal inflammation in injured rats. Given the established beneficial effects of 2-DG observed in other studies on neuronal activity and inflammation, adapted2-DG doses and/or longer treatment duration should be explored for reducing deleterious inflammatory processes occurring after SCI.