Study of human KIR family molecules with novel monoclonal antibodiesStudy of human KIR family molecules with novel monoclonal antibodies

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Presentation Time: 03:15 PM - 04:30 PM
Poster Board Number: B560
Abstract ID: 4155

Presenting Author:
Evan Teng , Scientist 1 at BioLegend

Abstract:

Killer Cell Immunoglobulin-like Receptors (KIR) is a critical family of receptors expressed by natural killer cells and T lymphocytes. 15 highly polymorphic KIR genes have been identified. Despite these receptors having been discovered decades ago, some of the receptors have not been well studied due to lack of effective research tools.  We recently developed a collection of monoclonal antibodies against the KIR receptor family members, including KIR2DL4, KIR2DL5, KIR2DS1, KIR2DS4, KIR2DS5, KIR3DL1, KIR3DL2, and KIR3DL3. The specificity of these antibodies has been thoroughly verified using transfected cell lines and human peripheral blood samples. For example, our KIR2DS1-specific monoclonal antibody can distinguish between the closely related KIR2DL1 versus KIR2DS1 proteins; our KIR2DL5-specific monoclonal antibodies can better target broader KIR2DL5 variants compared to current commercially available clones. In addition to those single specific KIR antibodies, multiple clones are able to determine the shared epitopes of two or more KIRs including KIR2DL2, KIR2DL3, KIR2DS2. We continue to further characterize these new monoclonal antibodies and are evaluating potential applications in immunophenotyping, diagnostics, and therapeutics.