Cytokine production by Group 3 Innate Lymphoid Cells requires innate antigen presenting cells and is regulated by TLR2.
Presentation Time: 03:15 PM - 04:30 PM
Poster Board Number: B622
Abstract ID: 4640
Presenting Author:
Julie Mirpuri , Associate Professor at Univ. of Texas Southwestern Med. Ctr.
Abstract:
Group 3 innate lymphoid cells (ILC3) can produce both IL-17 and IL-22 in the intestine. Neonatal mucosal ILC3 (nILC3) produce more IL-17 after maternal high fat diet (mHFD) exposure in vivo. The mechanisms underlying regulation of cytokine secretion in nILC3 are still not fully understood. We isolated neonatal intestinal lamina propria cells (nLP) from Rag-/- mice on regular chow (RD) and performed magnet enrichment for CD45+ve or pan-ILC cells. In vitro stimulation of CD45+ve enriched cells showed increased IL-17 (2-fold) and IL-22 (2-fold) production after PMA/Ionomycin stimulation by ELISA. In contrast, there was no increase in production of IL-17 and IL-22 after stimulation with ILC enrichment (depletes APCs), demonstrating that innate APCs are required for production of IL-17 and IL-22 by ILC3. In vitro stimulation of CD45+ve nLP cells from RD TLR2-/- mice demonstrated no increase of IL-22 and similar increase of IL-17 (2-fold) after stimulation when compared to RD Rag-/- nLP. Surprisingly, there was a 10-fold increase in IL-17 production in nLP after in vitro stimulation from HFD TLR2-/- mice, demonstrating that TLR2 is necessary for IL-22 production and suppression of IL-17 production in HFD nILC3. qRT-PCR of neonatal small intestine in TLR2-/- mice showed a comparable increase of IL-17 in mHFD offspring. This study identifies a mechanism for TLR2 mediated regulation of cytokine secretion by nILC3, which may be modified by APCs and dietary metabolites.
Cytokine production by Group 3 Innate Lymphoid Cells requires innate antigen presenting cells and is regulated by TLR2.
Category
Poster and Podium (Block Symposium)