Obese women have increased asthma prevalence and severity compared to lean women and lean and obese men. Our research showed that ovarian hormones increased Th2- and Th17-mediated airway inflammation and that there was a sex difference in Th2 and Th17 cell metabolism. Therefore, we hypothesize that ovarian hormones increase Th2 and Th17 metabolism and airway inflammation in high fat diet (HFD) mice. Sham-operated (S.O.) and ovariectomized (OVX) female mice were placed on control chow or HFD for 8 weeks. Mice were sensitized and challenged with house dust mite (HDM) to induce airway inflammation and lungs were harvested to assess infiltration of inflammatory cells. In separate experiments, spleens were harvested for in vitro differentiation of Th2 and Th17 cells to determine the mitochondrial respiration of these cells. After allergen challenge, female, S.O., HFD mice had increased numbers of lung Th2 and Th17 cells and increased lung IL-13 and IL-17A protein and circulating IgE expression compared to the OVX HFD mice and the S.O. lean female mice. Additionally, Th17 cells from HFD females had increased mitochondrial respiration compared to the Th17 cells from lean female mice in both S.O. and OVX female mice. No differences in glycolysis were seen in Th17 cells from any group. In conclusion, ovarian hormones increase Th17-mediated airway inflammation in HFD mice and HFD increases mitochondrial function of Th17 cells.