Presenting Author: Uryan Isik Can
, Postdoctoral Fellow at Natl. Jewish Hlth.
Abstract:
Group 2 innate lymphoid cells (ILC2s) are pivotal in type 2 immune response, crucial for allergies, defense against helminths, and tissue repair. Mainly residing in barrier tissues like the lung, intestine, and skin, ILC2s act as sentinels, detecting and responding to environmental signals. Lung ILC2s consists of tissue-resident natural ILC2s (nILC2s), and migratory inflammatory ILC2s (iILC2s) recruited during mucosal damage or infections. In helminth-induced lung injury, iILC2s emerge by day 5, becoming the early source of type 2 cytokines. However, their origin remains unclear. To explore lung, small intestine (SI), and bone marrow (BM) contributions to origins of iILC2s, we conducted a time-course analysis. Results indicate the lung and BM are unlikely major sources, while the SI remains a strong candidate. Additionally, photoconversion surgeries, of the SI or BM, using Kikume Green-Red mice to locally label and track cells showed that SI cells manifest in the lung as iILC2s, whereas BM cells appear to contribute to nILC2s. In support, adoptively transferred BM progenitors became nILC2s, as expected, but did not give rise to iILC2s. Lastly, scRNA sequencing for lung and mesenteric lymph node ILC2s and BM ILC2 progenitors corroborated our findings. In summary, (1) lung does not appear to be a prominent source for either nILC2s or iILC2s, (2) BM may serve as an origin for nILC2s, but it does not serve as a major source for iILC2s, and, (3) SI is the primary origin of iILC2s.
Unraveling the origins of recruited group 2 innate lymphoid cells
Category
Poster and Podium (Block Symposium)
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Date: May 5 Presentation Time: 03:15 PM to 04:30 PM Room: Exhibit Hall F1