Detecting immune activity in human tumors with novel high-throughput whole slide imaging approaches using Imaging Mass Cytometry.

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Presentation Time: 03:15 PM - 04:30 PM
Poster Board Number: B920
Abstract ID: 5449

Presenting Author:
Vinicius Motta , Field Applications Scientist at Standard BioTools

Abstract:

Deciphering the spatial biology of the tumor immune microenvironment (TIME) has potential to inform clinical and translational researchers about the likelihood of immunotherapy success. Imaging Mass Cytometry™ (IMC™) is a spatial biology imaging technique that enables deep characterization of the TIME while fully retaining spatial features of tissues using 40-plus markers simultaneously.

We developed two new whole slide imaging methods for IMC. Tissue mode (TM) rapidly acquires an image of the whole tissue that can be used for quantitative analysis of spatial biology. Preview mode (PM) samples the entire tissue at predefined spacing to rapidly capture a low-resolution image of all expressed markers, improving informed identification of areas for high-resolution cell mode (CM) imaging on the same slide.

Tumor tissues were stained using a 40-marker panel comprised of the Maxpar® Human Immuno-Oncology IMC Panel Kit, designed for tumor and immune cell profiling. TM imaging in combination with pixel-based analysis quantified tissue areas containing high or low abundance of myeloid and lymphoid immune, tumor and stromal cell compartments. PM in combination with CM imaging followed by single-cell analysis on selected tumor regions identified cell populations with varying activation states and expression profiles.  

Overall, we highlight the power of IMC to provide quantitative insights into the spatial biology of the TIME.

For Research Use Only. Not for use in diagnostic procedures.