Next-Level Efficiency: StraightFrom Spleen Isolation Kits - Standardizing Isolation of Untouched T cells with Minimal Hands-On Time

Isolation of T cells from mouse spleen is essential for various research fields. Studies using magnetically separated cells help to understand the immune system, develop new vaccines and advance immunotherapy. Thus, selecting a reliable cell isolation method is critical for consistent experimental results.

Simultaneous spleen dissociation and labeling of unwanted cells with an antibody cocktail was performed using the GentleMACS dissociator. Subsequent automated magnetic labeling of cells and isolation of untouched T cells (CD4, CD8 or Pan T) was performed using the autoMACS NEO Separator. Target cell purity, yield, cell activation status and functionality were assessed.

Untouched CD4, CD8 or Pan T cells obtained with our StraightFrom Spleen workflow were of the same high purity and yield as conventional more time-consuming protocols, which require preparation of single cell suspension and various centrifugation steps prior to enrichment. Flow cytometric analysis of isolated cells revealed no expression of the activation markers CD25 or CD69 in non-stimulated cells even after overnight cultivation. However, significant upregulation was observed after stimulation. In addition, isolated cells showed excellent proliferation in culture.

We have developed a simple and fast cell isolation method that significantly accelerates the isolation of functional cells, providing an attractive alternative to the laborious multi-step protocols used in academic and industrial laboratories.