StarBright Dyes — Simplifying Flow Cytometry with 8-color No Compensation Panels

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Presentation Time: 03:15 PM - 04:30 PM
Poster Board Number: B886
Abstract ID: 4597

Presenting Author:
Sharon Sanderson , Flow Cytometry Application Scientist and Product Manager at Bio-Rad Labs.

Abstract:

Flow cytometry is a powerful technique, allowing a vast amount of information to be obtained from a single sample. A well-designed panel is vital for good quality data. One aspect of panel design is to use fluorophore combinations that avoid close or overlapping spectra. If not, the panel will risk having high spillover, spreading, and reduced population resolution.

We have designed 8-color, no compensation panels for conventional flow cytometry where the fluorophores used have distinct spectra, therefore they have minimal signal into detection filters other than their own. This removes the need for post-acquisition compensation, saving time, as well as ensuring high resolution of cells due to the lack of spread. We show 8-color immunophenotyping panels, acquired on a 5-laser ZE5 Cell Analyzer, for human peripheral blood and mouse splenocytes which require no compensation. B cell, cytotoxic and memory T cell, natural killer cell, and monocyte populations were easily identified. These panels can also be used as a backbone for building larger panels by adding additional markers to detect other cells of interest.

Many antibodies incorporated into these panels, have been conjugated to StarBright^TM Dyes which have narrow excitation and emission profiles, making them ideal for inclusion in no compensation panels. StarBright Dyes also simplify panel design as they work with all buffers and most fixatives making them suitable for use in a wide range of protocols.