Presenting Author: Danny Murrow
, Undergraduate Researcher at Univ. of Cincinnati
Abstract:
A neuroimmune modulatory axis has been implicated as a crucial factor in the body’s physiological response to changing environmental stimuli. A major effector within this axis is thought to be the sympathetic nervous system, comprised of both adrenergic and purinergic communicability. Here, novel data illustrates the codetection of adenine-based purines and catecholamines measured using Fast-Scan Cyclic Voltammetry (FSCV) in murine mesenteric lymph nodes ex-vivo. FSCV is capable of both the spatiotemporal resolution necessary to measure rapid, spontaneous release of neurotransmitters and the sensitivity required to discriminate between purines and catecholamines. Our data uncover how the manipulation of a purinergic receptor alters catecholamine transmission. By blocking the activation of the G-protein-coupled receptor P2Y1, which is tacit in intracellular Ca2+ levels, we affected decreased concentration, greater duration, and lower frequency signaling. The bridge permitting cross cross-regulation is likely found in calcium-dependent exocytosis.