SUCNR1-mediated response in CD11b+CD11c+ macrophages and dendritic cells is essential for resistance to cutaneous leishmaniasis
Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B577
Abstract ID: 5976
Presenting Author:
Stella Francy de Assuncao , PhD candidate at Univ. de São Paulo, Fundação Oswaldo Cruz, Instituto Gonçalo Muniz Bahia
Abstract:
Cutaneous leishmaniasis (CL) is a neglected infectious disease prevalent in Brazil where it is mainly caused by L. braziliensis and L. amazonensis. The available treatments are chronic and can lead to resistance to the parasites, hence, we investigate the role of succinate receptor 1 (Sucnr1) in the disease given its inflammatory activity in DCs and MOs. We characterized SUCNR1 expression in the skin lesion of patients with CL, it is upregulated in the lesion and its expression is correlated with myeloid cell gene markers. To evaluate Sucnr1 role during CL, we infected WT and Sucnr1-/- mice with L. amazonensis and we observed an increase in the ear thickness, lesion size, edema and parasite burden in the absence of Sucnr1. The cytokine profile in the lesion shift in Sucnr1-/- mice with an increase in the infected CD11c+CD11b+ DCs and CD11c+CD11b+ MOs, they also presented a decreased intensity of iNOS expression. So we performed a killing assay with BMDM where we observed that Sucnr1-/- BMDM were unable to kill leishmania parasites. CD11ccreSucnr1f/f mice infected with L. amazonensis showed an increase in the ear thickness, lesion size, edema and parasite burden, they also presented increased infected MOs. To confirm that Sucnr1 expression by CD11c+ MOs is essential for controlling the parasites, we performed a killing essay with BMDM from Sucnr1f/f and CD11ccreSucnr1f/f and CD11ccreSucnr1f/f BMDM were unable to kill L. amazonensis.
SUCNR1-mediated response in CD11b+CD11c+ macrophages and dendritic cells is essential for resistance to cutaneous leishmaniasis
Category
Poster and Podium (Block Symposium)