Presenting Author: Pari Dhruva
, Graduate Researcher at Tulane Univ. Sch. of Med.
Abstract:
Aspergillus fumigatus is a ubiquitous mold that causes many lung diseases, the most severe of which is invasive pulmonary aspergillosis (IPA). Innate immunity is paramount for controlling IPA. Here, we examined the role of dendritic cells (DCs). Global depletion of DCs using CD11c-DTR mice results in impaired lung clearance of A. fumigatus, suggesting that one or more of these DC subsets plays a protective role. Unexpectedly however, we found that mice deficient in BATF3 (Batf3-/-), which lack type 1 conventional DCs (cDC1s), cleared A. fumigatus more efficiently than WT control mice at early time points (12 h and 24 h), suggesting that cDC1s play an inhibitory or non-protective role during A. fumigatus lung defense. Mechanistically, Batf3-/- displayed a hyper-type 17 response, which we have previously shown to be critical for eliminationof A. fumigatus from the lung. This correlated with differential IL-33 (negative for type 17, lower in Batf3-/-) vs. PGE2 (positive for type 17, higher in Batf3-/-) production, an axis we have reported that regulates type 17 responses. Overall, our data indicates that both protective and non-protective DC subsets are present in the lung during IPA.
The role of dendritic cell subsets in Invasive Pulmonary Aspergillosis
Category
Poster and Podium (Block Symposium)
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Date: May 4 Presentation Time: 11:30 AM to 12:45 PM Room: Exhibit Hall F1