Presenting Author: Masanori Matsumoto
, Assistant Professor at Univ. of Illinois, Urbana-Champaign
Abstract:
Staphylococcus aureus, a common pathogen in the skin, triggers skin diseases including atopic dermatitis and cutaneous abscesses. We recently found that neutrophils play differential roles in epicutaneous and intradermal S. aureus inoculation models. To understand the role of neutrophils in S. aureus-induced skin inflammation, neutrophil gene expression profiles were analyzed by single cell RNA-sequencing (scRNA-seq) analysis using those two infection models.
Methods: We infected C57BL6/J mice intradermally or epicutaneously with S. aureus USA300 strain and isolated neutrophils from the infected skin tissues followed by scRNA-seq analysis. To identify neutrophil genes that regulate skin inflammation, we generated specific gene deletion mice to investigate the underlying mechanisms.
Results: In the intradermal S. aureus infection model, we found that inducible nitrogen oxide synthetase (iNOS) gene expression was upregulated in neutrophils and that skin lesion size was significantly increased in neutrophil-specific iNOS-deficient mice. In the epicutaneous S. aureus inoculation model, however, the expressions of genes (padi4, mmp8/9, s100a8/9) required for neutrophil extracellular trap (NETs) were upregulated, and skin inflammation was suppressed in padi4-deficient mice.
Conclusion: These data suggest that neutrophil NO production is critical for dermal S. aureus killing, while NETs formation induces skin inflammation by epidermal S. aureus colonization.
Understanding the role of neutrophils in Staphylococcus aureus-induced skin inflammation
Category
Poster and Podium (Block Symposium)
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Date: May 5 Presentation Time: 11:30 AM to 12:45 PM Room: Exhibit Hall F1