Sulforaphane attenuates biomass smoke-induced inflammatory responses in murine- and human-derived cell lines.

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Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B625
Abstract ID: 5204

Presenting Author:
Dajanae Bivens , Integrated and Inclusive Science Post-baccalaureate Fellow

Abstract:

The combustion of organic matter, or biomass, is a common used energy source for heating and cooking, primarily in low and middle-income countries. Chronic exposure to combustion products results in inflammation and impairment of host defense mechanisms. Sulforaphane is a compound found within cruciferous vegetables that is reported to have potent anti-inflammatory and antioxidant properties. In this study, we aimed to determine if sulforaphane would mitigate the pro-inflammatory effects of wood smoke and acrolein exposure. RAW 264.7 murine macrophages and A549 human lung epithelial cells were pre-treated with sulforaphane and challenged with soluble wood smoke particles and acrolein.  A variety of molecular biology techniques were used to assess the pro-inflammatory and pro-oxidant effects of biomass smoke and the ability of sulforaphane to ameliorate these effects. The activation of intracellular kinases (ERK1/2, JNK, and Akt) was also assessed. Our data indicate that both acrolein and wood smoke induced a strong inflammatory response that was reduced with sulforaphane pre-treatment. Wood smoke and acrolein induced the expression of several genes, including COX-2, MCP-1, MIP-2/IL-8, HO-1, and Nrf-2. Data also suggest that sulforaphane reduces TLR agonist -induced inflammatory responses. Sulforaphane exhibited high cytotoxicity at higher concentrations. Our studies indicate that, at low concentrations, sulforaphane can attenuate biomass-smoke induced inflammatory responses.