Characterizing the mechanism of catecholamines transport in the Mesenteric lymph Node (MLN) using Fast Scan Cyclic Voltammetry (FSCV).

Neuroimmune communication is one of the key components in regulating the body’s response to physiological challenges and maintaining homeostasis while facilitating immune stress response. Within the gastrointestinal tract, the mesenteric lymph nodes play a key role in governing the immune response, and this regulation involves communication between immune cells and neural components.Sympathetic neurons are thought to co-release norepinephrine and dopamine to modulate cytokine signaling during inflammation and therefore measurements of these neurochemicals in the lymph node are critical for understanding immunity. Here, we have expanded our electrochemical waveform and way of performing the experiment in ex vivo lymph node slices to study how these neurochemicals communicate in the lymph node during different conditions. We have also tried blocking the different neurotransmitter transporter with different antagonist drugs for different catecholamines to see the differential change and came to know that these transport blockers have significant effect on holding the neurochemical in the extracellular space when we block their transporter.

This work presents the first-time detection on role of different transport blocker and their impact in the mechanism of catecholamine release in the MLN on a millisecond timescale, which will provide significant insight into the mechanisms of neuron-immune communication and different drug related studies.​