Ranked abundance of 115 proteins following simultaneous measurement by multiplex immunoassay in serum, plasma, and cell culture supernatant samples.

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Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B925
Abstract ID: 5586

Presenting Author:
Deborah Droll , Product Manager at MilliporeSigma

Abstract:

MILLIPLEX^® PLEXpedition, a customizable multiplex immunoassay kit, was developed to simultaneously screen up to 115 targets including cytokines, chemokines, growth factors, matrix metalloproteinases, and biomarkers of bone health, metabolism, and cardiovascular disease. Two Luminex^® instruments, the xMAP^® INTELLIFLEX^® and the FLEXMAP 3D^®, can accommodate all 115 bead regions with comparable sensitivity and sample correlation observed between them. The analyte selection incorporates many biomarkers critical to the field of immunology, using assays developed for two MILLIPLEX^® 48-plex human cytokine, chemokine, and growth factor kits (Cat. No. HCYTA-60K and Cat. No. HCYTB-60K). Sample correlation in serum, plasma, and cell culture supernatants demonstrated that this discovery biomarker panel with a matrix-free format can be used to transition seamlessly to our highly qualified panels, MILLIPLEX^® Human Cytokine Panel A and B, which have optimized serum matrix and lot-to-lot verification for absolute quantification of protein concentration. To probe the relative abundance of each analyte in typical sample matrices, the 115-plex assay was run with a set of 8 cell culture supernatants and 24 healthy and disease serum and plasma samples. Additionally, analytes were ranked by their response to stimulation by LPS or ConA in PBMCs. Our results highlight the utility of the novel PLEXpedition screening kit for evaluating relative abundance in biological samples.