Sample reinterrogation enables iterative development and real time analysis of high parameter spatial biology data

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Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B909
Abstract ID: 5235

Presenting Author:
John Schwarz , Research and Development Manager, Applications at Canopy Biosci.

Abstract:

Spatial biology offers immense power for immunology research. However, entering the field of spatial biology requires significant investments of both capital and time, as well as a commitment of high value patient samples. The design of robust assays is not trivial, and may require sample-specific adjustments. Here we present a novel solution that permits sample re-interrogation and mid-experiment panel adjustment in a spatial biology workflow.

The CellScape™ platform for Precise Spatial Multiplexing paired with VistaPlex™ Assay Kits provide a ready-to-use cyclic multiplex immunofluorescence (IF) solution to profile fresh frozen and FFPE tissue samples. Tissues are mounted in enclosed fluidic chambers, while immunofluorescence imaging is conducted on the CellScape instrument, divided into rounds of staining, image capture, and signal erasure. The researcher can pause between rounds and analyze the data in real time. To illustrate the benefit of this approach, we present a use case in which we applied VistaPlex antibody panels to reveal the spatial distribution of major immune cell populations. An analysis was completed after each round and conclusions were then drawn from this preliminary phenotype map, guiding decisions of whether and how to continue the experiment.

The CellScape sample reinterrogation feature is a pragmatic spatial biology solution that optimizes the use of precious resources, and best utilizes high value samples that are limited or irreplaceable.