StarBright Dyes — Expanding the Availability and Choice of Fluorophores to Improve Immunophenotyping Data in Veterinary Studies

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Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B887
Abstract ID: 4598

Presenting Author:
Sharon Sanderson , Flow Cytometry Application Scientist and Product Manager at Bio-Rad Labs.

Abstract:

Flow cytometry is an important application for veterinary studies that is commonly used for diagnostics, drug screening, and for the fundamental understanding of veterinary diseases. However, the range of fluorophores that flow cytometry veterinary antibodies are conjugated to is limited. This restricts the choice available for multiplexing panels and therefore panel size, which is especially important for rare samples as the information that can be determined from a single sample will be reduced. In addition, less choice means that best practice for panel design cannot always be followed, often resulting in poor cell resolution and data quality.

To address this problem, Bio-Rad is expanding the range of antibodies that are conjugated to StarBright^TM Dyes, to include canine, bovine, and porcine targets. Antibodies conjugated to StarBright Dyes are bright, easy to use, and will work with most protocols and cytometers. This means you have the choice and flexibility to follow best practice and make bigger panels, getting better results, saving time and resources.

We present data showing multiplexing panels on canine, bovine, and porcine peripheral blood, acquired on the ZE5 Cell Analyzer from Bio-Rad. These immunophenotyping panels include antibodies which are conjugated to traditional fluorophores as well as new StarBright Dyes. We demonstrate how multiple cell populations and subpopulations can easily be detected, such as B cells, T cells, and natural killer cells.