Sargassum horneri ethanol extract containing polysaccharides and phenolic acids attenuates PM-induced intrinsic apoptotic cell death via ROS scavenging and transition metal chelation
Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B861
Abstract ID: 5336
Presenting Author:
Hyo Jin Kim
Abstract:
Particulate matter (PM) is recognized a cause of respiratory diseases resulting from cellular oxidative damage and, ultimately, apoptosis. Sargassum horneri, an edible brown alga, has been traditionally used as food and medicine. In this study, we analyzed the physicochemical characteristics of S. horneri ethanol extract (SHE) and investigated its role against PM-induced apoptosis in type II alveolar epithelial cell, MLE-12. SHE polysaccharide was composed of high mannuronic acid (69%) and its molecular weight and polydispersity were 5.6×104g/mol and 2.5. Also, the main phenolic acid of SHE was gallic acid complex. We uncovered that SHE chelated transition metals (Si, Mg, Al, Ti, and Fe) on the outer surface of MLE-12 cells which, when uncleared, triggered intracellular ROS generation and lipid peroxidation. PM-induced oxidative stress further produced oxidative DNA damage and eventually led to apoptotic cell death through intrinsic pathway. However, SHE not only attenuated the intracellular ROS and lipid peroxidation but also restored PM-induced oxidative DNA damage and apoptosis in MLE-12 cells. The anti-ROS/lipid peroxidation effect of SHE turned out to be similar to that of N-acetyl-L-cysteine (NAC), a commonly used reactive oxygen species (ROS) scavenger, which completely reversed the PM-mediated oxidative DNA damage. Collectively, our results suggest that antioxidant ability of SHE suppressed apoptosis by restraining PM-induced oxidative DNA damage.
Sargassum horneri ethanol extract containing polysaccharides and phenolic acids attenuates PM-induced intrinsic apoptotic cell death via ROS scavenging and transition metal chelation
Category
Poster