Maternal Lactobacillus johnsonii supplementation reduces allergic responses in offspring by impacting its gut microbiota and functional innate immune responses
Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B855
Abstract ID: 4961
Presenting Author:
Llilian Arzola Martinez , Postdoc Fellow at Michigan Med., Univ. of Michigan
Abstract:
Allergies are driven by Th2 immune responses developed in atopic individuals following exposure to allergens. Microbiota dysbiosis of mucosal sites have been associated with allergic disorders possible due to its immunomodulatory potential. Our prior research revealed that Lactobacillus johnsonii (Lj) oral supplementation protects against viral and allergen airway exposure by influencing immune cells, particularly dendritic cells. To further investigate whether Lj supplementation of moms could alter offspring responses to allergic disease, female mice received Lj supplementation for seven days before mating. The resulting offspring were sensitized to cockroach allergen at 5 weeks after birth. Offspring from Lj-supplemented mothers exhibited reduced airway hyperreactivity and a diminished Th2 immune response. Additionally, the 5w old offspring from Lj treated moms showed a more consistent cecal microbiota with increased Verrucomicrobia phylum bacteria, known for improving immune responses. Finally, bone marrow derived dendritic cells from 5w old offspring from Lj-supplemented mothers differentiated in vitro and stimulated with respiratory syncytial virus for 24 hours showed decreased production of proinflammatory innate cytokines and chemokines. These results have shown the immunomodulatory potential of prenatal Lj supplementation to protect from allergic immune responses in distal mucosal surfaces by altering offspring’s gut microbiota and systemic immune cell responses.
Maternal Lactobacillus johnsonii supplementation reduces allergic responses in offspring by impacting its gut microbiota and functional innate immune responses
Category
Poster