Immune dynamics in response to risk group 4 pathogens in nonhuman primate models

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Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B829
Abstract ID: 5582

Presenting Author:
Deja FP Rivera , Immunology Research Associate at NIAID, DCR, IRF-Frederick, NIH

Abstract:

Despite the critical importance of emerging infectious disease research, the underlying immune responses to risk group 4 pathogens remain largely understudied relative to many other pathogens. Spectral flow cytometry has dramatically increased the capacity of fluorescence-based cell analyses, allowing for the development of comprehensive panels across a variety of cell types. We have developed a 33-color phenotypic panel that incorporates a plethora of markers to simultaneously identify numerous distinct cellular populations within circulation and in tissue with a deliberate and extensive characterization of monocytes, dendritic cells, and tissue-resident T cells. This panel utilizes multiple approaches to accurately identify appropriate cell markers that can be easily distinguished with minimal to no compensation, despite containing fluorochromes with similar excitation and emission spectra. Importantly, this high-complexity panel was implemented on both fresh and frozen samples of whole blood, peripheral blood mononuclear cells (PBMCs), and cells isolated from multiple peripheral tissues across several nonhuman primate (NHP) models and on frozen human PBMCs, making this a broad cross-reactive panel enabling direct comparisons among NHP species and with humans. This new phenotypic flow panel allows for broad insight into underlying immune responses to infections across species while remaining amenable to additional potential markers of interest.