Identification of anti-human and mouse mAb cross-reactive to agriculture and wildlife species

From the spread of zoonotic disease to ensuring a safe food supply, animal health is a key component of “One Health”; however, reagents necessary to monitor immune status in agricultural and wildlife species are lacking compared to those available for humans and rodents. Thus, we evaluated the cross-reactivity of commercially available human and mouse cell surface mAb panels via flow cytometry to cells from pigs, cattle, and white tailed-deer (WTD). Specifically, PBMCs from pigs, cattle, and WTD as well as single cell spleen and lymph node suspensions (pig and cattle only) were evaluated. A minimum of 11 antibodies were cross-reactive with each species/panel combination, with a median of 28 antibodies per species/panel, and a maximum of 52 cross-reactive mAbs (human mAbs to WTD).  More human mAbs were cross-reactive to porcine and bovine cells than mouse mAbs (approximately 50% more human mAbs cross-reacted), but interestingly, the same proportion of cross-reactive mAbs (21%) was observed to WTD PBMCs for both the human and mouse panels. For example, anti-human CD11a and CD11b reacted against WTD PBMCs, while multiple B cell subset mAbs were cross-reactive to porcine PBMCs and tissue. Research to further validate specificity and cross-reactivity of these mAbs with indicated species is ongoing. Identification of commercially-available, fluorescently-labeled mAbs cross-reactive to livestock and wildlife immune cells will enhance relevant research programs across the globe.