Serological and Cellular Kinetics of the Antibody Light Chain Response to Influenza A and B Viruses in Ferrets

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Presentation Time: 11:30 AM - 12:45 PM
Poster Board Number: B1033
Abstract ID: 5141

Presenting Author:
Robert A Richardson , Pre-doctoral Fellow at Florida Res. & Innovation Ctr., Cleveleand Clin., Univ. of Georgia

Abstract:

A past publication indecated that the primary antibody response of ferrets to influenza A H1N1 virus (H1N1 IAV) hemagglutinin (HA) is predominantly governed by λ-light chain immunoglobulin (Igλ), whereas the secondary response to an antigenically distinct H1N1 IAV HA shifts to κ-light chain immunoglobulin (Igκ) dominance. To further assess this observation, ferrets were infected with either H1N1 IAV, H3N2 IAV, B/Yamagata-lineage IBV, or B/Victoria-lineage IBV and bled for serum 1, 3, 5, 7, 10, 14, 21, and 28 days post-infection (dpi). The serum were tested for its ability to bind the homologous HA to the challenge virus with an ELISA. This presentation will go over recently generated data that extents the observation of the Igλ  light chain bias to B/Yamagata and B/Victoria lineage IBV primary infections as well as determines the Igλ serological kinetics during IAV and IBV primary infections. Ferrets primarily infected with B/Yamagata-, B/Victoria-lineage IBV, and H3N2 IAV had a significate Igλ bias by 7 dpi and those primarily infected with H1N1 IAV had a significate Igλ bias by 10 dpi. Finally, the Igλ bias was present up to 28 dpi in all of the groups. The purpose of this research is to establish a fundamental understanding of the ferret humoral immune response to IAV and IBV. In summary, the data extends our knowledge the Igλ bias during primary immune response to IBV primary infections as wells as determined that the bias arises 7-10 dpi and is present at 28 dpi.